The complex hexaploid wheat genome offers many challenges for genomics research. Expressed sequence tags facilitate the analysis of gene-coding regions and provide a rich source of molecular markers for mapping and comparison with model organisms. The objectives of this study were to construct a high-density EST chromosome bin map of wheat homoeologous group 2 chromosomes to determine the distribution of ESTs, construct a consensus map of group 2 ESTs, investigate synteny, examine patterns of duplication, and assess the colinearity with rice (...) of ESTs assigned to the group 2 consensus bin map. A total of 2600 loci generated from 1110 ESTs were mapped to group 2 chromosomes by Southern hybridization onto wheat aneuploid chromosome and deletion stocks. A consensus map was constructed of 552 ESTs mapping to more than one group 2 chromosome. Regions of high gene density in distal bins and low gene density in proximal bins were found. Two interstitial gene-rich islands flanked by relatively gene-poor regions on both the short and long arms and having good synteny with rice were discovered. The map locations of two ESTs indicated the possible presence of a small pericentric inversion on chromosome 2B. Wheat chromosome group 2 was shown to share syntenous blocks with rice chromosomes 4 and 7. (shrink)

DBS Think Tank IX was held on August 25–27, 2021 in Orlando FL with US based participants largely in person and overseas participants joining by video conferencing technology. The DBS Think Tank was founded in 2012 and provides an open platform where clinicians, engineers and researchers can freely discuss current and emerging deep brain stimulation technologies as well as the logistical and ethical issues facing the field. The consensus among the DBS Think Tank IX speakers was that DBS expanded in (...) its scope and has been applied to multiple brain disorders in an effort to modulate neural circuitry. After collectively sharing our experiences, it was estimated that globally more than 230,000 DBS devices have been implanted for neurological and neuropsychiatric disorders. As such, this year’s meeting was focused on advances in the following areas: neuromodulation in Europe, Asia and Australia; cutting-edge technologies, neuroethics, interventional psychiatry, adaptive DBS, neuromodulation for pain, network neuromodulation for epilepsy and neuromodulation for traumatic brain injury. (shrink)

A total of 1918 loci, detected by the hybridization of 938 expressed sequence tag unigenes from 26 Triticeae cDNA libraries, were mapped to wheat homoeologous group 4 chromosomes using a set of deletion, ditelosomic, and nulli-tetrasomic lines. The 1918 EST loci were not distributed uniformly among the three group 4 chromosomes; 41, 28, and 31% mapped to chromosomes 4A, 4B, and 4D, respectively. This pattern is in contrast to the cumulative results of EST mapping in all homoeologous groups, as reported (...) elsewhere, that found the highest proportion of loci mapped to the B genome. Sixty-five percent of these 1918 loci mapped to the long arms of homoeologous group 4 chromosomes, while 35% mapped to the short arms. The distal regions of chromosome arms showed higher numbers of loci than the proximal regions, with the exception of 4DL. This study confirmed the complex structure of chromosome 4A that contains two reciprocal translocations and two inversions, previously identified. An additional inversion in the centromeric region of 4A was revealed. A consensus map for homoeologous group 4 was developed from 119 ESTs unique to group 4. Forty-nine percent of these ESTs were found to be homoologous to sequences on rice chromosome 3, 12% had matches with sequences on other rice chromosomes, and 39% had no matches with rice sequences at all. Limited homology was found between wheat ESTs on homoeologous group 4 and the Arabidopsis genome. Forty-two percent of the homoeologous group 4 ESTs could be classified into functional categories on the basis of blastX searches against all protein databases. (shrink)

The objectives of this study were to develop a high-density chromosome bin map of homoeologous group 7 in hexaploid wheat, to identify gene distribution in these chromosomes, and to perform comparative studies of wheat with rice and barley. We mapped 2148 loci from 919 EST clones onto group 7 chromosomes of wheat. In the majority of cases the numbers of loci were significantly lower in the centromeric regions and tended to increase in the distal regions. The level of duplicated loci (...) in this group was 24% with most of these loci being localized toward the distal regions. One hundred nineteen EST probes that hybridized to three fragments and mapped to the three group 7 chromosomes were designated landmark probes and were used to construct a consensus homoeologous group 7 map. An additional 49 probes that mapped to 7AS, 7DS, and the ancestral translocated segment involving 7BS also were designated landmarks. Landmark probe orders and comparative maps of wheat, rice, and barley were produced on the basis of corresponding rice BAC/PAC and genetic markers that mapped on chromosomes 6 and 8 of rice. Identification of landmark ESTs and development of consensus maps may provide a framework of conserved coding regions predating the evolution of wheat genomes. (shrink)

Commentary on the famous Buddhist Sutra of the wise and the foolish (Mdzangs blun zhes bya baʼi mdo).

'Thick neural event' is introduced to mean an event that requires firings of more than one neuron and a substantive (i.e. additional to merely temporal and spatial) relation among them. It is shown that some well regarded theories (e.g. by Lamme, Koch, etc.) strongly suggest that neural correlates of consciousness (NCCs) are thick neural events. It is then shown that thin (= not thick) neural events provide sufficient causation for neural events leading to behaviour, and that there are good reasons (...) to reject overdetermination by thick events. Thus, any physicalism that recognizes legitimacy of a search for NCCs must either reject theories that embrace thickness of NCCs, or accept the epiphenomenalistic view that the properties in virtue of which we are conscious are not properties in virtue of which events causally contribute to our behaviour. (shrink)

Heidegger’s characterization of Dasein as Being-in-the-world suggests a natural relation to environmental philosophy. Among environmentalists, however, closer inspection must raise alarm, both since Heidegger’s approach is in some senses inescapably anthropocentric and since Dasein discovers its environment through its usability, serviceability, and accessibility. Yet Heidegger does not simply adopt a traditionally modern, instrumental view. The conditions under which the environment appears imply neither that the environment consists only of tools, nor that what is true of the parts is also true (...) of the whole, nor that an orientation to use—where appropriate—precludes any other orientation. Heidegger’s anthropocentric commitments thus do not rule out the possibility of a non-instrumental perspective on the natural world. (shrink)

As a method of oppositional reading, deconstruction argues that a text, and by extension any object of observation including the self, is characterized by disunity rather than unity. The present paper proposes that if we define the self as having a dimension that is not an object of observation, but is a pure witness, or what in Eastern cultures is known as ‘pure consciousness’, then deconstruction can be seen to undo in practice what it claims to do in theory. This (...) reversal has implications for the postmodernist self, which is thought to be fragmented by a multiplicity of social voices and the loss of a unifying depth of feeling. Through an analysis of the deconstructive notions of consciousness and language, this paper suggests that fragmentation can in effect take the postmodernist self toward a sense of wholeness. In theory deconstruction undermines the unity of language and consciousness, while in practice it invites a nonconceptual response similar to that of aesthetic experience. The deconstructive ‘freeplay’ of language empties out the meaning of a text and leads the reader toward a state of being anterior to thought, toward an experience of awareness itself as opposed to its phenomenal content. (shrink)

by W. S. F. Pickering Durkheim's sociological approach to morals and moral systems has always aroused considerable interest, be it by way of criticism or ...

In two shocking articles that appeared in 1968 and 1974, Garrett Hardin argued that the population explosion was producing a “tragedy of the commons.” Since we lack an effective method of sharing common resources, the strong incentive for individuals to appropriate them selfishly would soon lead to their collapse. To mitigate this danger, Hardin proposed a “lifeboat ethic”: less populated and -polluted Western countries should deny food aid to developing nations, where it would save lives only to increase population pressure, (...) and they should close their borders to immigration to prevent their lifeboats from becoming overcrowded and going down with the rest. This paper challenges and complicates Hardin’s account of the tragedy. While there is something right about his view, its vulnerability to a series of empirical challenges reflects its conceptual limitations. I argue that we need to develop a broadly ethical and arguably religious solution to the twin challenges of population growth and pollution. If the liberal commitment to negative freedom is, ironically, largely responsible for our current ecological bind, our only hope of escape is to build bridges between traditions in search of a thicker sense of ethicopolitical obligation. (shrink)

Originally presented as author's thesis (Ph. D.--Madurai Kamaraj University).