Recombinant adeno‐associated viruses (rAAVs) are promising vectors for the delivery of various genetic constructs into eukaryotic cells. rAAVs have a number of properties that make it possible to successfully use them both in vitro and in vivo. Purification and concentration of rAAV vectors are critical for achieving high viral titer, stability, efficiency, and purity. This review systematically analyses all available purification approaches. The purification methods described in this work differ substantially from each other in mechanisms, efficiency, labor time, and cost. (...) Researchers have to choose a purification algorithm depending on the purpose of their work. We strive to simplify the choice of the necessary and sufficient technique based on the experimental needs and available resources of the laboratory. (shrink)

Cellular 3D structures, for example, organoids, are an excellent model for studying and developing treatments for various diseases, including hereditary ones. Therefore, they are increasingly being used in biomedical research. From the point of view of safety and efficacy, recombinant adeno‐associated viral (rAAV) vectors are currently most in demand for the delivery of various transgenes for gene replacement therapy or other applications. The delivery of transgenes using rAAV vectors to various types of organoids is an urgent task, however, it is (...) associated with a number of problems that are discussed in this review. Cellular heterogeneity and specifics of cultivation of 3D structures determine the complexity of rAAV delivery and are sometimes associated with low transduction efficiency. This review surveys the main ways to solve emerging problems and increase the efficiency of transgene delivery using rAAVs to organoids. A clear understanding of the stage of development of the organoid, its cellular composition and the presence of surface receptors will allow obtaining high levels of organoid transduction with existing rAAV vectors. (shrink)

Kinetic demixing in quaternary mixed oxides has not been investigated previously, either theoretically or experimentally. In this theoretical study, we derive simple and exact analytical expressions that describe the steady-state cation concentration profiles resulting from kinetic demixing in mixed oxides of the O type. The concentration profiles are expressed in terms of cation-vacancy exchange frequency ratios. We assume a random distribution of cations and make use of the Moleko-Allnatt exact sum-rule expression relating the phenomenological coefficients in the ternary random alloy (...) to the vacancy mechanism operative. We employ Monte Carlo simulation of kinetic demixing to verify the result of the analysis. We discuss how kinetic demixing profiles can be processed to obtain atom-vacancy exchange frequency ratios. We also discuss how atom-vacancy exchange frequencies themselves might be obtained from available diffusivities in the quaternary oxide. (shrink)